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2013.5.21 Synthetic Yeast Chromosome XII and beyond

Junbiao DAI

Associate professor, School of Life Sciences, Tsinghua Univeristy



2006-2011      Postdoctoral fellow, Department of                              Molecular Biology and Genetics, Johns                        Hopkins University, School of Medicine
2000-2006      Ph.D. , Department of Genetics,                                          Development and Cell Biology, Iowa State                        University
1997-2000      M.S. , Department of Biological Science                              and Biotechnology, Tsinghua University
1993-1997      B.S., Department of Intensive Instruction,                        Nanjing University







Time:1:00pm, May. 21, 2013


Address:Rm. 102, Old Chemistry Building, east Wing, 1rd floor, CQB



Recent advances in DNA synthesis technology have enabled the construction of novel genetic pathways and genomic elements, furthering our understanding of system-level phenomena. The ability to synthesize large segments of DNA allows the engineering of pathways and genomes according to arbitrary sets of design principles. I will describe the international synthetic yeast genome project (Sc2.0), and the effort to synthesize the first million-base-pair eukaryotic chromosome: the Saccharomyces cerevisiae chromosome XII. The synthetic genome features several systemic modifications, including an inducible evolution system termed SCRaMbLE (Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution).   We show the utility of the SCRaMbLE system as a novel method of genome alteration, capable of generating complex genotypes and a broad variety of phenotypes and genotypes.  Detected alterations include deletions, inversions, and duplications.  The fully synthetic yeast genome will allow restructuring of the yeast genome on an unparalleled scale, and will open the door to a new type of combinatorial genetics based on variations in gene content and copy number, rather than base changes.

Host:Fangting LI