Title: CRISPR technology for genome regulation and applications

Speaker： Dr. Lei Stanley Qi

              Assistant Professor, Departments of Bioengineering, Chemical and Systems Biology, and Stanford ChEM-H, Stanford University

Time： 10:00am June 4th 2015

Address： Rm 101, East wing of Old Chemistry Building, Peking Unversity 

Chair：  Dr. Ping Wei,  Center for Quantitative Biology 

Abstract：

       Gene circuits allow programmable activation and repression of the genome. This provides a novel and powerful for both understanding biology and disease modeling. Here I will talk about our work on repurposing the bacterial CRISPR system as a synthetic biology toolset for sequence-specific gene regulation. The CRISPR tools enable multiplexable, inducible, and high-throughput activation or repression of genes across diverse organisms. The technology also allows genome-wide perturbation of gene expression for functional genomic research. We show several applications using the basic CRISPR tools, e.g., the construction of higher-order CRISPR circuits for modulating metabolic pathways in yeast. The CRISPR technology provides a powerful approach to engineering complex genetic systems for studying gene function and genomics.