定量生物学中心

学术报告

题目：  Detection and Manipulation of Single Biomolecular Machines

报告人： 刘诗欣 博士

             Assistant Professor，Head of the Laboratory of Nanoscale Biophysics and Biochemistry，The Rockefeller University

时间：2015-6-8（周一），13:00-14:00

地点：北京大学老化学楼东配楼101报告厅

主持人：定量生物学中心，魏平 研究员

摘要:

After twenty-five years of development, single-molecule techniques have become popular tools to study biology. There are two broad families of single-molecule approaches: fluorescence-based detection and force-based manipulation. I will present two systems to demonstrate the power of these approaches in dissecting the molecular mechanism of complex biological processes. First, optical trapping experiments showed that a bacteriophage genome packaging motor simultaneously rotates and translocates the DNA genome during the viral life cycle. This homomeric ring ATPase displays a remarkable level of coordination and an unexpected division of labor among its subunits. As DNA is packed into the viral capsid and the internal pressure rises, the motor gradually changes its step size and the amount of DNA rotation in order to achieve robust and efficient packaging. Second, single-molecule FRET assays revealed that the HIV reverse transcriptase is able to flip and slide on nucleic acid substrates. These large-scale motions allow the enzyme to quickly switch between various binding modes and perform different functions.

报告人简介：刘诗欣，2003年获中国科技大学生物学学士学位，2009年被授予哈佛大学化学博士学位，之后于加州大学伯克利分校从事博士后研究，2015年被聘为洛克菲勒大学助理教授。曾在Cell，Science，NSMB，eLife，PNAS等学术期刊发表论文。曾获中国国家优秀留学生奖和美国国家卫生研究院K99奖。