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教授，博士生导师 电话：010-62754743 实验室主页：http://www.phy.pku.edu.cn/~pkuluocx/index.htm 办公地址： 物理大楼办公中308房间, 微流与纳米技术中心北452，454房间, 综合科研楼431房间，435-437房间 2001年7月毕业于北京大学物理学院 学士学位； 2006年7月毕业于北京大学物理学院 博士学位。 |
担任职务：
北京大学物理学院教授；
北京大学定量生物学中心教授。
研究方向：
微纳加工，微流控，芯片实验室，高通量平台，定量生物学。
我们的研究兴趣主要集中利用新方法定量系统的研究细胞对微环境的响应行为，包括细菌的趋化性，抗药性，细菌种群协作，细胞胁迫应激反应，细胞迁移等方面。
研究兴趣：
微流控技术，微环境及微环境变化对细胞行为的影响。
工作经历：
2006 年 7 月 -2008 年 6 月，北京大学物理学院博士后；
2008 年 7 月-2011年5月，北京大学前沿交叉科学研究院特聘研究员；
2011年5月-2017年7月,北京大学物理学院副教授；
2017年8月至今，北京大学物理学院教授。
项目情况：
2006年-2010年作为科研骨干参与国家自然科学基金重点项目 “微流的物性与流动规律”
2007年-2010年主持自然科学基金青年项目"微流体系中的纳升液滴间的反应控制"
2012年-2015年 主持自然科学基金面上项目"生命体在时空变化微环境下的响应行为的定量研究",同时得到国家自然科学基金创新群体，介观物理重点实验室经费支持。
2015年-2019年，作为科研骨干参与国家自然科学基金重点项目“细胞迁移动力学研究”
参与介观物理重点实验室自主课题
2019.7-2024.6 科研骨干参与重大专项“ 生物元器件标准化设计组装与应用”
2020.1-2023.12 主持自然科学基金面上项目“ 应用微流系统研究癌细胞力化耦合迁移以及干细胞分化过程中的粘弹性质变化路径”
授课情况：
定量生物学实验与技术基础 课程负责人，每年秋季开学，3学分，授课地点，综合科研楼434，435，436房间
系统生物学选讲 授课教师， 每年春季
理论与系统生物学Seminar 授课教师，每年秋季
普通物理实验 授课教师，每年春季
其他工作
Applied Physics letters, Lab on a chip，Biomicrofluidics等杂志审稿人。定量生物中心微流平台负责人，国家凤凰工程北大高通量细胞分析平台负责人。
发表论文：
72. Xiao Li, Jialin Shi, ZiqingGao, Jian Xu, ShujingWang, Xin Li, Qi Ouyang, Chunxiong Luo*, Biophysical studies of cancer cells’ traverse-vessel behaviors under different pressures revealed cells’ motion state transition Scientific Reports , 2022, 12:7392, 通讯作者.
71. Xiao Li,Yiteng Jin,Jialin Shi,Xiaoqiang Sun,Qi Ouyang,Chunxiong Luo*,A high throughput microfluidic system with large ranges of applied pressures for measuring the mechanical properties of single fixed cells and differentiated cells, Biomicrofluidics, 2022, 16, 034102 , 通讯作者.
70. Jing Zhang, Wenting Shen, Zhiyuan Cai, Kaiyue Chen, Qi Ouyang, Ping Wei, Wei Yang*, Chunxiong Luo*, Microfluidic-Enabled Multi-Cell-Densities-Patterning and Culture Device for Characterization of Yeast Strains’ Growth Rates under Mating Pheromone, Chemosensor, 2022, 10(4), 141, 通讯作者.
69. Fengyu Zhang, Yanhong Sun, Yihao Zhang, Wenting Shen, Shujing Wang, Qi Ouyang, Chunxiong Luo*, Independent control of amplitude and period in a synthetic oscillator circuit with modified repressilator, Communications Biology, 2022, 5, 23, 通讯作者.
68. Kaiyue Chen#, Wenting Shen#, Ziqing Gao, Chunxiong Luo*, Stress response capacity analysis during aging and possible new insights into aging studies, Current Genetics, 2021, 67(3):1-4
67. Fengyu Zhang#, Yanhong Sun#, Chunxiong Luo*, Microfluidic approaches for synthetic gene circuits' construction and analysis, Quantitative Biology，2021, 9 (1): 47-60
66. Chunyang Lu#, Jian Xu#, Jintao Han, Xiao Li, Ningtao Xue, Jinsong Li, Wenhua Wu, Xinlei Sun, Yugang Wang, Qi Ouyang, Gen Yang*, Chunxiong Luo* , A novel microfluidic device integrating focus-separation speed reduction design and trap arrays for high-throughput capture of circulating tumor cells, Lab on a Chip, 2020, 20,4094-4105 , 通讯作者.
65. Ziqing Gao, Jian Xu, Kaiyue Chen, Shujing Wang, Qi Ouyang and Chunxiong Luo*，Comparative analysis of yeast replicative lifespan in different trapping structures using an integrated microfluidic system, Advanced Materials Technologies，2020, 5(12):2000655, 通讯作者.
64. Kaiyue Chen, Nan Rong, Fangzheng Xiong, Qi Ouyang, Chunxiong Luo*，A novel two-layer-integrated microfluidic device for high-throughput yeast proteomic dynamics study at single cell level, Integrative Biology，2020，12(10) 241-249，通讯作者
63. Kaiyue Chen, Wenting Shen, Zhiwen Zhang, Fangzheng Xiong, Qi Ouyang, Chunxiong Luo*, Age-dependent decline in stress response capacity revealed by protein dynamics analysis, Scientific reports，2020，10:15211，通讯作者
62. Hongcun Zhu, Yeping Cui, Chunxiong Luo, Feng Liu*, Quantifying Temperature compensation of Bicoid gradients with a fast T-tunable microfluidic device, Biophysical Journal, 2020, 119,1-11.
61. Jian Xu, Xudong Wang, Xiao Li, Gen Yang, Chunxiong Luo*, High-throughput cell migration assay under combinatorial chemical environments by a novel 24-well-plate based device. Biomedical Microdevices , 2020, 22, 40，通讯作者
60. Lingjun Li, Tian Wu, Ying Wang, Min Ran, Yu Kang, Qi Ouyang, Chunxiong Luo*, Spatial coordination in mutually beneficial bacterial community enhances its antibiotic resistance. Communications Biology，2019， 2： 301， 通讯作者
59. Xing Shi#， Changjun Shao#, Chunxiong Luo#， Yanan Chu, Jian Wang, Qingren Meng, Jun Yu, Zhancheng Gao, Yu Kang，Microfluidics-Based Enrichment and Whole-Genome Amplification Enable Strain-Level Resolution for Airway Metagenomics.mSystem, 2019, 4（4）e00198-19，共同一作
58. Xiang Zhao, Chunxiong Luo*, Hongli Wang*, Protein dynamic analysis of the budding yeast sporulation process at the single-cell level in an air-enriched microfluidic device. Integrative Biology , 2019， 11（3） 79-86.通讯作者
57. Xuanqi Zhang, Guangwei Si, Yiming Dong, Kaiyue Chen, Qi Ouyang, Chunxiong Luo*, and Yuhai Tu*, Escape band in Escherichia coli chemotaxis in opposing attractant and nutrient gradients, PNAS, 2019, 116(6) 2253-2258. 通讯作者
56. Tingting Li, Yiming Dong, Zhang, Xuanqi Zhang, Ji, Xiangyu Ji, Chunxiong Luo， Chunbo Lou， Haoqian Zhang, Qi Ouyang,Engineering of a genetic circuit with regulatable multistability, Integrative Biology, 2018, 10， 474-482.
55. Leqian Yu, Junjun Li, Jiayin Hong...Chunxiong Luo, ...Li Liu, Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells, Stem cell reports,2018, 11, 142–156.
54. Xiaoqiang Sun, Shaorong Li， Shujing Wan， Chunxiong Luo*， Benxiang Hou*， The evaluation of E-faecalis colonies dissolution ability of sodium hypochlorite in microenvironment by a novel device. Biomedical Microdevices,2018,20, 36. 通讯作者
53. Tian Wu, Changjun Shao, Lingjun Li,Shujing Wang, Qi Ouyang,Yu Kang*， Chunxiong Luo*,Streamline-based purification of bacterial samples from liquefied sputum utilizing microfluidics, Lab on a chip，2017，17, 3601-3608. 联系作者，背封面文章
52. Bin Shao, Haiyu Yuan, Rongfei Zhang,Xuan Wang, Shuwen Zhang, Qi Ouyang, Nan Hao*, Chunxiong Luo*，Reconstructing the regulatory circuit of cell fate determination in yeast mating response. Plos Computational Biology, 2017,13:e1005671.联系作者
51. Zhaojun Li, Qiuxian Cai, Xuanqi Zhang, Guangwei Si, Qi Ouyang, Chunxiong Luo*, and Yuhai Tu*，Barrier Crossing in Escherichia coli Chemotaxis， Physical Review Letters ,2017，118, 098101.联系作者
50. Kai Wei, Minghao Qiu, Rongfei Zhang, Liantong Zhou, Ting Zhang, Maosheng Yao*, Chunxiong Luo*,Single Living yEast PM Toxicity Sensor (SLEPTor) System, Journal of Aerosol Science, 2017, 107, 65-73. 联系作者
49. Rongfei Zhang, Haiyu Yuan, Shujing Wang, Qi Ouyang, Yong Chen, Nan Hao, Chunxiong Luo*， High-throughput single-cell analysis for the proteomic dynamics study of the yeast osmotic stress response， Scientific reports,2017,DOI: 10.1038/srep42200， 联系作者
48. Qianghua Quan, Shuwen Zhang, Xudong Wang, Qi Ouyang, Yugang Wang, Gen Yang * , Chunxiong Luo * , A parallel and quantitative cell migration assay using a novel multi-well-based device.Biomedical microdevices, 2016, 18, 99. 联系作者
47. Xiaoqiang Sun, Shujing Wang, Yue Kang, Chunxiong Luo*, Benxiang Hou*, Study of invasion and colonization of E. Faecalis in microtubes by a novel device, Biomedical Microdeivces, 2016, 18,82-89,联系作者
46. Haiyu Yuan, Rongfei Zhang, Bin Shao, Xuan Wang, Qi Ouyang, Nan Hao and Chunxiong Luo*; Protein expression patterns of the yeast mating response, Integrative Biology, 2016； 712-719, 联系作者，正封面文章
45. Qiuxian Cai, Zhaojun Li, Qi Ouyang, Chunxiong Luo* and Vernita D.Gordon*;Singly Flagellated Pseudomonas aeruginosa Chemotaxes Efficiently by Unbiased Motor Regulation, mBio, 2016,doi: 10.1128/mBio.00013-16.联系作者
44. Xuanqi Zhang, Lingjun Li, Chunxiong Luo*； Gel integration for microfluidic applications, Lab Chip, 2016,16, 1757-1776. 联系作者 ，综述文章
43. Wei Yang, Chunxiong Luo*, Luhua Lai, and Qi Ouyang*, A novel microfluidic platform for studying mammalian cell chemotaxis in different oxygen environments under zero-flow conditions, Biomicrofluidics,2015, 9, 044121,联系作者
42. Min Ran,Ying Wang, Sida Wang, Chunxiong Luo*,Pump-free gradient-based micro-device enables quantitative and high-throughput bacterial growth inhibition analysis,Biomedical Microdevices,2015, 17: 67, 联系作者
41. Ying Wang, Min Ran, Jun Wang, Qi Ouyang*, Chunxiong Luo*, Studies of Antibiotic Resistance of Beta-Lactamase Bacteria under Different Nutrition Limitations at the Single-Cell Level, PLoS ONE ,2015, 10(5): e0127115, 联系作者
40. Xuejun Zhu, Xin Ge, Ning Li, Long-Fei Wu, Chunxiong Luo, Qi Ouyang, Yuhai Tu and Guanjun Chen, Angle sensing in magnetotaxis of Magnetospirillum magneticum AMB-1,Integrative Biology , 2014, 6 , 706-713.
39. Xianjie Kang, Lingli Jiang, Xi Chen, Haiyu Yuan, Chunxiong Luo*, Qi Ouyang*, Pump-free multi-well-based microfluidic system for high-throughput analysis of size-control relative genes in budding yeast, Integrative Biology , 2014, 6 , 685-693. 联系作者
38. Weikang Wang, Yi Quan , Qibin Fu, Yu Liu, Ying Liang, Jingwen Wu, Gen Yang, Chunxiong Luo , Qi Ouyang , Yugang Wang, PLOS ONE, 2014, 9(1), e84654, 10.1371/journal.pone.0084654.
37. Xiaorong Wang # , Yu Kang # , Chunxiong Luo # , Tong Zhao, Lin Liu, Xiangdan Jiang, Rongrong Fu, Shuchang An, Jichao Chen, Ning Jiang, Lufeng Ren, Qi Wang, Kenneth Baillie, Zhancheng Gao * , and Jun Yu * ， Heteroresistance at Single Cell Level: Adapting Antibiotic Stress by Population-based Strategy and Growth-controlled Inter-phenotypic Coordination, mBio, 2014, 5(1) : e00942-13. 并列第一作者.
36. Xiangdan Jiang, Yu Kang, Xingjie Pan, Jun Yu, Qi Ouyang, Chunxiong Luo* , Studies of drug resistance response of sensitive and drug-resistance strains in microfluidic system, Integrative Biology , 2014, 6:143-151( Inside front cover story) . 联系作者.
35. ShuangYu Bi, Daqi Yu, Guangwei Si, Chunxiong Luo,Tongqing Li, Qi Ouyang, Jakovljevic, Vladimir, Sourjik, Victor, Yuhai Tu, Luhua Lai, Discovery of novel chemoeffectors and rational design of Escherichia coli chemoreceptor specificity, PNAS,2013,110:16814-16819.
34. Yuan Tian, Chunxiong Luo* , Qi Ouyang * , A microfluidic synchronizer for fission yeast, Lab on a Chip, 2013, 13 (20), 4071 - 4077. 联系作者.
33. Wei Yang, Zhaojun Li,Weilin Zhang, Chunxiong Luo*,Qi Ouyang, Gen Yang,Yugang Wang,A novel density control device for the study of cancer cell autocrine effect,Biomedical Microdevices,2013, 15:683-689, 联系作者
32. Sisi Li, Jian Shi, Li Liu, Junjun Li, LM Jiang, Chunxiong Luo , Kamei K, Yong Chen , MICROELECTRONIC ENGINEERING , 2013 ,110, 70-74 .
31. Xianjie Kang, Chunxiong Luo * , Qiong Wei, Chunyang Xiong , Qian Chen, Ying Chen, Qi Ouyang*, Mass production of highly monodisperse polymeric nanoparticles by parallel flow focusing system, Microfluidics and Nanofluidics ,2013, 15:337-345, 联系作者
30. Xuejun Zhu, Guangwei Si, Nianpei Deng, Qi Ouyang, Tailin Wu, Zhuoran He, Lili Jiang, Chunxiong Luo *, and Yuhai Tu* ,Frequency-dependent Escherichia coli chemotaxis behaviors, Physical Review Letters ,2012,108,128101, 联系作者
29. Guangwei Si , Wei Yang , Shuangyu Bi , Chunxiong Luo* and Qi Ouyang, A parallel diffusion-based microfluidic device for bacterial chemotaxis analysis, Lab on a Chip , 2012, 12 , 1193-1197， 联系作者.
28. Yuan Tian , Chunxiong Luo* , Yuheng Lu , Chao Tang and Qi Ouyang*, Cell cycle synchronization by nutrient modulation ,Integrative Biology 2012 ， 4 ， 328-334, 联系作者
27. Zhengwei Xie, Yi Zhang, Ke Zou, Onn Brandman, Chunxiong Luo, Qi Ouyang and Hao Li, Molecular phenotyping of aging in single yeast cells using a novel microfluidic device, Aging cell, 2012, 11, 599–606.
26. Yi Zhang,Chunxiong Luo, Ke Zou, Zhengwei Xie, Onn Brandman, Qi Ouyang, Hao Li, Single Cell Analysis of Yeast Replicative Aging Using a New Generation of Microfluidic Device, PLOS ONE, 2012,7(11), e48275, DOI:10.1371/journal.pone.0048275.
25. Gen Yang, Yi Quan, Weikang Wang, Qibin Fu, Jingwen Wu, Tao Mei, Jia Li, Yubo Tang, Chunxiong Luo, Qi Ouyang, Shaopeng Chen, Lijun Wu, Tom K. Hei, and Yugang Wang, Dynamic equilibrium between cancer stem cells and non-stem cancer cells in human SW620 and MCF-7 cancer cell populations. British Journal of Cancer; 106: 1512-9; 2012.
24. Guowei Lu, Lei Hou , Tianyue Zhang, Jie Liu, Hongming Shen , Chunxiong Luo , Qihuang Gong, JOURNAL OF PHYSICAL CHEMISTRY C, 2012 ,110, 25509-25516
23. Guowei Lu, Jie Liu, TianYue Zhang, Wenqiang Li, Lei Hou , Chunxiong Luo , Franck Lei, Michel Manfait , Qihuang Gong,NANOSCALE , 2012 , 4, 3359-3364
22. Wang, QY; Lu, GW; Hou , L ; Zhang,; Luo , CX ; et.al, CHEMICAL PHYSICS LETTERS , 2011, 503,256-261.
21. Chunxiong Luo, et.al,Differentiating stem cells on patterned substrates for neural network formation, 2011,Microelectronic Engineering 8, 1707-1710 第一作者
20. Ning Li, Chunxiong Luo, et.al.Microfluidic generation and dynamically switching of oxygen gradients applied to the observation of cell aerotactic behavior, 2011, Microelectronic Engineering 8,1698-1701
19. Chunxiong Luo # , Xiaofang Ni # , Li Liu, Shin-ichiro M. Nomura, Yong Chen*, Degassing-assisted patterning of cell culture surfaces, Biotechnology and Bioengineering , 105,2010, 854-859. 并列第一作者
18. Li Liu # , Chunxiong Luo # , Xiaofang Ni, et.al., A micro-channel-well system for culture and differentiation of embryonic stem cells on different types of substrate, BIOMEDICAL MICRODEVICES 12,2010, 505-511, 并列第一作者
17. Guangwei Si, Xuejun Zhu, Yangsen Kang, Chunxiong Luo *, Qi Ouyang*, Yong Chen,Diffusion-based concentration control in microcavities during long time period by programmed syringe pumps, Microelectronics Engineering 87 , 793-797 2010 联系作者
16. Tao Zhu#, Chunxiong Luo#, Jianyong Huang, Chunyang Xiong, Qi Ouyang and Jing Fang,Electroporation based on hydrodynamic focusing of microfluidics with low dc voltage, Biomedical Microdevices, 12,2010, 35-40. 并列第一作者
15. Xianjie Luo, YangShen Kang, Chunxiong Luo*, et al.,An automatic microturbidostat for bacterial culture at constant density,BIOMEDICAL MICRODEVICES 12: 499-503, 2010, 联系作者
14. Li, H; Lei, L; Zeng , Q; Shi, Jian ; Luo , CX ; Ji , H; Ouyang , Q ; Chen, Y, SENSORS AND ACTUATORS B-CHEMICAL , 2010, 145,570-574.
13. Hao Li, Chunxiong Luo, Hang Ji, Qi Ouyang and Yong Chen,Micro-pressure sensor made of conductive PDMS for microfluidic applications, Microelectronic Engineering , 87(2010), 1266-1269.
12. Wang, L ; Ni, XF ; Luo , CX ; Zhang, ZL ; Pang, DW ; Chen, Y, BIOMEDICAL MICRODEVICES , 2009 ,11, 679-684.
11. Chunxiong Luo , Lingli Jiang, Shibo Liang, Qi Ouyang*, Hang Ji and Yong Chen,High-throughput microfluidic system for monitoring diffusion-based monolayer yeast cell culture over long time periods , Biomedical Microdevices , 11, 2009, 981-986. 第一作者
10. Chunxiong Luo , Xuejun Zhu, Tao Yu, XianjiaLuo, Qi Ouyang, Hang Ji, Yong Chen.A fast cell loading and high-throughput microfluidic system for long-term cell culture in zero-flow environments. Biotechnology and Bioengineering , 2008 ， 101,190-195. 第一作者
9. Chunxiong Luo , Hao Li, Chunyang Xiong, Xiaoling Peng, Qingli Kou , Yong Chen, Hang Ji , and Qi Ouyang,The combination of optical tweezers and microwell array for cell manipulation and cultivation in microfluidic device. Biomedical Microdevices , 2007, 9, 573-578. 第一作者
8. Wang, Z. M.; Bao, K.; Xu, L. P.; Luo, C. X.; Zhang, B.; Ji, H.; Quyang, Q.; Chen, Y., Microfluidic cooling of semiconductor light emission diodes, MICROELECTRONIC ENGINEERING,2007, 1223-1226.
7. Wang, Z. M.; Luo, X. J.; Wang, S.; Luo, C. X.;Sun, M. H.; Bao, K.; Zhang, B.; Zhang, G. Y.; Wang, Y. G.; Chen, Y. ; Ji, H.; Ouyang, Q., Light output enhancement of a GaN-based light emitting diode by polymer film imprinting, SEMICONDUCTOR SCIENCE AND TECHNOLOGY, 2007,22, 279-282.
6. Chunxiong Luo , Xiaojing Yang, Qiang Fu, Manhui Sun, Qi Ouyang, Yong Chen, Hang Ji,Picoliter-volume aqueous droplets in oil: electrochemical detection and Yeast cell electroporation, Electrophoresis , 27 (2006) 1977-1983. 第一作者
5. Chunxiong Luo, Qiang Fu, Hao Li, Luping Xu, Manhui Sun, Qi Ouyang, Yong Chen and Hang Ji,PDMS microfludic device for optical detection of protein immunoassay using gold nanoparticles, Lab on a Chip , 5, (2005)726-729. 第一作者.
4. Chunxiong Luo, Manhui Sun, Luping Xu,Zhiling ZHANG, C. Crozatier, Yong CHEN ，and Hang Ji, Controllable PH gradient forming in microfluidic chips，Chin. Sci. Bull., 50 (2005) 629-631
3. Manhui Sun, Chunxiong Luo, Xu LP, Ji H, Qi OY, Yu DP & Chen Y. Artificial lotus leaf by nanocasting. LANGMUIR 2005, 21 (19): 8978-8981
2. Youdong Mao, Chunxiong Luo & Qi Ouyang. Studies of temperature-dependent electronic transduction on DNA hairpin loop sensor.Nucleic Acids Res 2003, 31, e108.
1. Youdong Ma, Chunxiong Luo , Wei Deng, Guanyin Jin, Xiaomei Yu, Zhaohui Zhang, Qi Ouyang, Runsheng Chen & Dapeng Yu. Reversibly Swithable DNA Nanocompartment on Surface. Nucleic Acids Res. 2004, 32, e144.
国家专利：
一种细胞趋化分析芯片及装置，授权公告号：CN203320000U，授权公告日：2013.12.04， 专利发明人 :罗春雄，杨薇，冉敏，欧阳颀一种多孔单细胞观测板及其应用 , 授权公告号：CN102796659B， 授权日：2014.06.11，专利发明人 : 罗春雄 , 蒋灵俐 , 杨薇 , 姜香丹
一种可布置不同细胞密度的微流控芯片及其应用，授权公告号：CN102796667B，授权日：2014.07.30, 专利发明人：罗春雄，杨薇，李昭君，康显阶
一种用于高通量细胞迁移研究的方法和细胞迁移研究系统（授权号 ZL 201610617882.5 ），申请人：罗春雄，张荣飞，张书文，杨根，王宇钢，欧阳颀 授权日2018.07.6
